Project objectives (i) Complete characterisation of putative fungal genes isolated from the cDNA library prepared Q from Sicala V-1 plants infected with V. dahliae. (ii) Analyse the functional role of potential fungal pathogenicity genes and conduct surveys to identify genetic polymorphisms in other V. dahliae isolates that might be associated with increased pathogenicity. (iii) Conduct survey of V. dahliae isolates using AFLP fingerprinting to identify genetic polymorphisms that might be associated with increased pathogenicity. (iv) Develop a molecular assay for the differentiation of pathogenic strains in field material.